Klotho (alphaKlothoLR) (20mcg)

Origin & Classification

Klotho is a transmembrane protein with a soluble form produced by proteolytic cleavage. It serves as a co-receptor for FGF23 and modulates phosphate and calcium homeostasis. The alphaKlothoLR variant is a recombinant long-release construct that fuses the extracellular domain of Klotho to an albumin-binding peptide via a linker. This design prolongs the protein’s half-life in circulation by reversible binding to serum albumin. Produced in mammalian cell systems, alphaKlothoLR is a glycosylated protein belonging to the class of recombinant endocrine regulators.

Mechanism & Research Context

In its role as an FGF23 co-receptor, Klotho is involved in regulating phosphate excretion, vitamin D metabolism, and parathyroid hormone secretion. It also exerts endocrine effects by modulating insulin/IGF-1 signaling, oxidative stress responses, and Wnt pathways. Researchers use alphaKlothoLR to study prolonged modulation of these pathways, enabling investigation into how sustained Klotho activity affects mineral balance, vascular calcification, cognitive function, and aging processes. Studies focus on downstream signaling cascades such as ERK1/2, PI3K/AKT, and FOXO transcription factors, as well as cross-talk with inflammatory mediators. The long-release design allows analysis of chronic Klotho exposure in cell and animal models without implying clinical applications.

Composition & Quality

AlphaKlothoLR is supplied as a lyophilized or liquid sterile formulation. Production under GMP-aligned conditions includes mammalian cell culture, purification through affinity and size-exclusion chromatography, and formulation with stabilizers. Quality control covers glycosylation profiling, SDS-PAGE, Western blot, binding affinity assays, endotoxin testing, and verification of albumin binding. Purity typically exceeds 95 %, and certificates of analysis document structural characteristics, potency, and storage conditions. The protein is stored at low temperature and reconstituted carefully to preserve bioactivity.

Summary & Unified Disclaimer

This description summarizes the scientific context of the molecule. It is provided for educational and research discussion purposes only. It is not intended to diagnose, treat, cure, or prevent any disease, and no medical or consumption claims are made.